一定剂量X线照射对大鼠脊髓损伤模型组织结构及功能恢复的影响

A certain dosage of X-irradiation can enhance the functional and structural recovery of the injured spinal cord of rats

目的探讨一定剂量X线照射对大鼠压迫型脊髓损伤模型组织结构及功能恢复的影响。方法采用动脉瘤夹建立大鼠压迫型脊髓损伤模型,70只SpragueDawley大鼠均接受T2节段的脊髓损伤,然后被随机分为2组,照射组在损伤后14d接受X线照射,对照组则不接受任何治疗。所有大鼠分别在脊髓损伤后第3、7、14、21、28、35和42天接受后肢运动及斜板测定,43d将所有实验大鼠处死后取出脊髓损伤标本,进行组织学检查。结果70只实验鼠中符合设计要求的共62只,其中照射组32只,对照组30只。X线照射组大鼠的后肢运动恢复及斜板测定均明显优于对照组,两者之间的差异有统计学意义(P<0.01)。照射组大鼠脊髓损伤部位的水肿及坏死区域明显少于对照组,胶质原纤维酸性蛋白(glialfibrillaryacidicprotein,GFAP)阳性细胞数明显少于对照组,而神经元数目则明显多于对照组。结论一定剂量X线照射可以改善脊髓损伤区的组织结构并促进大鼠脊髓损伤后运动功能的恢复。

Objective To study whether a certain dosage of X-irradiation can enhance the functional and structural recovery of the injured spinal cord of rats. Methods The compressive model of spinal cord injury of rats was set up by clip compression at the T 2 level, then 70 Sprague-Dawley rats were randomly divided into two groups. The experimental group received X-irradiation 14 days after injury, the control group did not receive X-irradiation. The functional tests were performed at day 3, 7, 14, 21, 28, 35, and 42 after irradiation including open field movement and inclined plane. All injured rats were sacrificed 43 days after injury and the injured spinal cords were taken out for histological tests. Results 62 rats met the experimental requirements among 70 injured rats, 32 rats in experimental group and 30 rats in control group. Statistically significant difference was achieved between two groups in open field movement and inclined plane (P<0.01). The edema and necrosis area of injured spinal cords of experimental group were less than those in control group, the positive number of glial fibrillary acidic protein of experimental group was less than those in control group, and the axons of experimental group were more than those in control group. Conclusions A certain dosage of X-irradiation can improve the structural recovery of the injured spinal cord and to enhance the functional recovery of the injured rats.