蛋白质降解与死亡时间推断的初步研究

A Study on the Relationship between the Degradation of Protein and the Postmortem Interval

目的观察大鼠肝脏肌动蛋白、微管蛋白在死后不同时间的降解情况,为死亡时间的推断寻找客观依据。方法大鼠麻醉致死后置于21℃温度控制系统模拟死后18d的改变,在不同时间点剪取肝脏组织抽提蛋白质,利用westernblot技术检测肌动蛋白、微管蛋白的降解变化并对产物进行半定量分析。结果大鼠肝脏肌动蛋白在死后8d尚可检测,死亡10d后检测不到;在死后2d,α微管蛋白已检测不到,但可检测到β微管蛋白,死亡4d后,β微管蛋白也检测不到。结论肌动蛋白、微管蛋白的死后降解存在差异,其在肝脏组织保存时间的不同可作为死亡时间推断的参数。

Objective To observe the degradation of actin and tubulin in the liver tissue of rats after death and to find an objective indicator of the postmortem interval(PMI). Methods Female rats were killed under anesthesia by ether and incubated at 21℃ in a temperature controlled system to simulate postmortem changes for 18 days postmortem. Protein in the hepatic tissue was extracted, actin and tubulin were then examined by western blot. Thereafter, the semi-quantitative analysis of the image of western blot was performed. Results Actin in the liver tissue of rats could be detected at 8 days postmortem, but could not be examined after 10 days postmortem. β-tubulin rather than α-tubulin could be examined after 2 days postmortem, and β-tubulin could not be examined at 4 days postmortem. Conclusion There is some difference in the degradation between actin and tubulin, their different preservation period postmortem may be used as a parameter for PMI estimation.