回转器模拟失重对SGC-7901细胞增生和周期的影响

Effects of rotating clinostat simulated weightlessness on cell proliferation and cell cycle of human gastric carcinoma cell line SGC-7901

目的:探讨模拟失重对胃癌SGC-7901细胞的细胞增生和细胞周期的影响.方法:采用回转器模拟失重,用免疫组化法观察SGC-7901细胞PCNA表达的变化,并用流式细胞仪测定其细胞周期的变化.结果:模拟失重后12,24,36h回转组PCNA抗体阳性细胞比率与对照组相比无明显差异,48,72h回转组的PCNA抗体阳性细胞明显减少(P<0.05及P<0.01).12,24,36h回转组的细胞周期与对照组相比无明显差异,48,72h后G0+G1期细胞显著增多(P<0.01及P<0.05),S+G2+M期细胞则显著减少(P<0.01及P<0.05).结论:回转器模拟失重抑制SGC-7901细胞的增生,抑制细胞G1期向S期的转化.

AIM: To investigate the effect of simulated weightlessness on cell cycle and proliferation of human gastric carcinoma cell line SGC-7901. METHODS: A rotating clinostat was used to simulate weightlessness. The expression of PCNA was examined by immunohistochemical staining. The changes of cell cycle were examined by flow'cytometry. RESULTS: The expression of PCNA did not change significantly after 12 h, 24 h, and 36 h of simulated weightlessness, but decreased significantly after 48 h and 72 h (P<0.05 and P<0.01, respectively). The cell cycle of 12 h, 24 h, and 36 h groups did not change significantly. However, the cells in G0+G1 phase in 48 h and 72 h groups were increased significantly (P<0.01 and P<0.05, respectively), while the cells in S+G2+M phase were decreased significantly (P<0.01 and P<0.05). CONCLUSION: Simulated weightlessness by a clinostat can prevent the transition of cells from G1 phase to S phase, and thus the proliferation of SGC-7901 cells was inhibited.