水蓑衣提取物对裸鼠肝癌移植瘤生长的影响

Effects of extraction from Hygrophila salicifolia on hepatocarcinoma cell xenografts in nude mice

目的:观察水蓑衣提取物的抗肝癌细胞增生活性,为水蓑衣提取物用于肝癌治疗提供实验依据.方法:BALB/CA雌性裸鼠24只,背侧皮下接种SMMC-7721人肝癌细胞建立荷瘤裸鼠模型,14d后随机分为对照组,低剂量水蓑衣组,高剂量水蓑衣组和羟基喜树碱组;给药20d后检测各组移植瘤的体积和质量;并观察移植瘤的组织学变化和血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)活性和肌酐(CRE)水平.结果:与对照组相比,水蓑衣组裸鼠移植瘤的体积明显减小(P<0.05),质量显著减轻(P<0.05);低、高剂量水蓑衣组的抑瘤率分别为46%和62%(P<0.05).水蓑衣组裸鼠瘤组织病理改变与羟基喜树碱组相似;体质量和血清ALT,AST,LDH活性及CRE与羟基喜树碱组无明显差别.结论:水蓑衣提取物可显著抑制肝癌移植瘤的生长,且呈一定的剂量依赖性:其对动物整体状况及心、肝、肾功能的影响与羟基喜树碱相当.

AIM: To assess the anti-proliferative effect of the extraction from Hygrophila salicifolia on hepatocellular carcinoma (HCC) cells, and to provide the experimental evidence for the use of Hygrophila salicifolia extraction in the therapy of hepatocarcinoma. METHODS: Twenty-four female nude mice were subcu-taneously injected with human HCC cells. After 14 days, the mice were divided equally into control group, low dose Hygrophila salicifolia group, high dose Hygrophila salicifolia group and hydroxycamptothecin group. The volume and weight of tumor were measured 20 days after treatment. Histological changes of the xenografts and the serum levels of AST, ALT, LDH, and CRE were determined. RESULTS: In comparison with the control group, the tumor volume and weight of both Hygrophila salicifolia groups were decreased significantly (P<0.05). The tumor inhibiton rates were 46% and 62% (P<0.05) in low- and high- dose group, respectively. Histological examination results from Hygrophila salicifolia groups were almost the same as those from hydroxycamptothecin group. There were no obvious difference in the weights and the serum levels of AST, ALT, LDH, CRE the mice treated with Hygrophila salicifolia and those treated with hydroxycamptothecin. CONCLUSION: The extraction from Hygrophila salicifolia can significantly inhibit the growth of hepatocarcinoma cell xenografts in nude mice in a dose-dependent manner. Its effects on the general condition and on the functions of the heart, the liver and the kidney are almost the same as those of hydroxycamptothecin.