摘要
根据已有文献及公开的拟南芥基因组序列,利用PCR方法从拟南芥(Arabidopsisthaliana)基因组DNA中扩增得到了转录因子CBF4上游的DNA片断,对其进行序列分析表明与GenBank序列高度同源,同时对其进行初步的功能分析。采用生物信息学方法对这一序列分析的结果显示这一片段具有启动子的特殊结构域;与GUS基因融合构建双元表达载体,转化烟草的组织特异性表达检测可见明显的GUS活性,初步表明所获片断为CBF4基因的诱导型启动子。
Transcriptional factors play an equivalent role in plant during drought adaptation and cold acclimation. In order to study the expression pattern of CB F4 gene promoter and explore its potential application in plant genetic engineer ing, CBF4 promoter fragment was amplified by PCR method from Arabidopsis thalian a genome DNA. The sequence analyses show it shares 99% homology with GenBank seq uence at nucleotide level. This sequence was also analyzed with related Bio-Info rmatics software in the Internet. Binary expressing vector was constructed by in serting the promoter sequence to upstream of GUS gene in pBI121, resulting in th e replacement of original CaMV35S promoter by the CBF4 promoter. GUS histochemic al expression analysis indicated that CBF4 promoter was an induced promoter and could confer an expression of GUS gene.
出处
《分子植物育种》
CAS
CSCD
2005年第3期363-368,共6页
Molecular Plant Breeding
