脑性瘫痪儿疾病静止期血清白细胞介素6、肿瘤坏死因子α水平与健康儿童的比较

Comparison of serum levels of interleukin-6 and tumor necrosis factor-alpha between children with cerebral palsy at quiescent period and healthy children

目的:检测脑性瘫痪(脑瘫)儿疾病静止期血清白细胞介素6、肿瘤坏死因子α水平,并与健康儿童相比较。方法:对象来源于2001-01/2002-12吉林大学第一医院的28例脑瘫儿(脑瘫组)及健康体检儿童15例(对照组),采取静脉血应用酶联免疫吸附试验法测定其血清白细胞介素6、肿瘤坏死因子α水平,并进行两组间的比较。结果:按意向处理分析,①脑瘫组和对照组血清白细胞介素6、肿瘤坏死因子α检测的阳性率基本相似61%(17/28),54%(15/28);47%(7/15),53%(8/15)。②脑瘫组血清白细胞介素6的含量明显高于对照组(41.16±10.52)ng/L,(30.05±2.52)ng/L,t=3.93,P<0.05;肿瘤坏死因子α含量明显高于对照组(37.37±11.84)ng/L,(21.98±3.75)ng/L,t=4.28,P<0.05。结论:脑瘫儿及正常儿童血清中均存在白细胞介素6和肿瘤坏死因子α,在疾病静止期测得白细胞介素6和肿瘤坏死因子α水平显著增高。脑瘫儿确实存在免疫调节机制紊乱,其细胞因子在脑瘫发病机制中的可能作用尚需进一步探讨。

AIM: To detect the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in children with cerebral palsy (CP) at quiescent period, and compare the results with those of healthy children. METHODS: The subjects were 28 CP children (CP group) and 15 healthy children (control group), who came for physical examination, in the First Hospital of Jilin University between January 2001 and December 2002.Venous blood of the subjects in both groups was drawn to detect the serum levels of IL-6 and TNF-αwith enzyme-linked immunosorbent assay (ELISA), and the results were compared between the two groups. RESULTS: According to intention-to-treat (ITT) analysis, ①The positive rates of serum IL-6 and TNF-αwere generally similar in the CP group [61%(17/28), 54%(15/28)] and control group [47%(7/15), 53%(8/15)]. ②The serum contents of IL-6 and TNF-αin the CP group [(41.16 ±10.52), (37.37±11.84) ng/L] were both obviously higher than those in the control group [(30.05±2.52), (21.98±3.75) ng/L] (t=3.93, 4.28, P< 0.05). CONCLUSION:IL-6 and TNF-αexist in the serum of both CP children and normal children, and the IL-6 and TNF-αlevels of CP children at quiescent period are significantly increased, which suggests that there is disorder in the mechanism of immunoloregulation in CP children.The possible role of cytokines in the pathogenesis of CP still needs further exploration.