蜂毒活性肽对大鼠佐剂性关节炎的作用(英文)

Bioactive peptide from bee venom for adjuvant-induced arthritis in rats

背景:传统上用蜜蜂粗毒治疗风湿性关节炎、类风湿性关节炎等,但有很强副作用,人们一直希望能从蜂毒中筛选出具有药效的单一组份。目的:探讨蜂毒活性肽是否可以通过调节机体免疫功能而抑制关节炎,为类风湿关节炎治疗探索一条新路。设计:以实验动物为研究对象的完全随机设计,对照实验研究。单位:一所市级动物生物学重点实验室。材料:实验于2001-01/2002-05重庆市动物生物学重点实验室完成。取Wistar雄性大鼠80只,2～3月龄,体质量180～200g,清洁级,由解放军第三军医大学动物实验中心提供。实验动物合格证号为SYXK1(军)2002-007。动物共分为3组:正常对照组(10例),关节炎组(10例),活性肽用药组(30例)。方法:利用大鼠佐剂性关节炎动物模型,肌肉注射活性肽,观察关节体积、关节指数的变化情况。主要观察指标:蜂毒活性肽对关节炎动物模型大鼠关节体积及关节指数的影响。结果:给药10d后,用药组(0.15mg/只)的关节体积为(4.72±0.58)mL和关节指数为4.47±0.46,与对照组比较有明显差异(P<0.05)。结论:活性肽对大鼠佐剂关节炎具有一定的抗炎作用,在临床上它可能具有的应用潜力。

BACKGROUND:Traditionally,bee venom can treat rheumatic arthritis,rheumatoid arthritis(RA) and so on,but it has strong side effects.So it has been hoped for a long time that the effective angle component could be screened from bee venom,which can be used for the treatment of arthritis perfectly than bee venom. OBJECTIVE:To investigate whether bioactive peptide from bee venom could inhibit infection of arthritis by regulating immunological function so as to probe into a new treatment for RA DESIGN:Completely randomized controlled experimental trial based on experimental animals METHODS:A municipal key laboratory of animal biology. MATERIALS:The experiment was carried out in the Chongqing Key Laboratory of animal biology from January 2001 to May 2002.Totally 80 rats of clean grade aged 2 to 3 months old with the body mass of 180 to 200 g were provided by Animal Experiment Center of Third Military Medical University of Chinese PLA.The experimental animal certification number was SYXK1(army) 2002-007.The animals were divided into 3 groups:normal control group(10 cases),arthritis group(10 cases),bioactive peptide group(30 cases). METHODS:Adjuvant-induced arthritis animal models were used and bioactive peptide were given to the animals by muscle injection to observe the knuckle volume and knuckle index changes. MAIN OUTCOME MEASURES:The effect of bioactive peptide from bee venom on the change of knuckle volume and knuckle index in adjuvant-induced arthritis rats RESULTS:Ten days after injection of 0.15 mg for each rat ,the volume of the paw was (4.72±0.58)mL and the knuckle index was (4.47±0.46)mL,which there was significant difference compared with the control group (P< 0.05). CONCLUSION:P-peptide possibly has certain inhibitory effect on the development of the adjuvant-induced arthritis in Wistar rat,and will possibly be a potential therapeutic drug.