卵巢肿瘤MMP-2、MMP-9及TIMP-2和细菌L型检测的意义

Expression of MMP-2、MMP-9 、TIMP-2 and infection of bacterial L-forms in ovarian neoplasms and its clinical significance

目的:探讨MMP-2、MMP-9及TIMP-2和细菌L型感染在卵巢肿瘤中的表达及临床意义。方法:采用免疫组化和革兰染色方法检测12 0例卵巢肿瘤中的MMP-2、MMP-9及TIMP-2表达,以及细菌L型的检出率,并用χ2 检验进行统计学处理。结果:卵巢乳头状癌中MMP-2、MMP-9及TIMP-2的表达率均明显高于良性肿瘤(P <0 .0 0 5 )。MMP 2、MMP 9在卵巢乳头状癌中临床分期Ⅲ、Ⅳ期中的表达率明显高于Ⅰ、Ⅱ期,随着病理分级增高而显著增加(P <0 .0 0 5 ) ,腹腔淋巴结有转移和有腹腔积液者均高于无腹腔淋巴结转移和无腹腔积液者(P <0 .0 5～P <0 .0 0 5 )。而TIMP 2阳性表达与MMP 2、MMP 9阳性表达相反(P <0 .0 5～P <0 .0 0 5 )。细菌L型检出率在恶性肿瘤与良性肿瘤差异无显著性(P >0 .0 5 ) ,但随病理分级、临床分期增高而显著增加(P <0 .0 0 5 ) ,腹腔淋巴结有转移和有腹腔积液者均明显高于无腹腔淋巴结转移和无腹腔积液者(P <0 .0 0 5 )。结论:MMP-2、MMP-9及TIMP-2在卵巢肿瘤中不同程度异常表达及细菌L型检出率与肿瘤的临床分期、病理分级、有无腹腔淋巴结转移及有无腹腔积液者均有明显关系,L型感染可能为诱发肿瘤因素之一,MMP- 2、MMP-9与L型感染可能有协同致瘤作用。

Objective:To study the expression of MMP-2、MMP-9、TIMP-2 and infection of bacterial L-forms in ovarian neoplasms and to discuss the clinical significance.Methods:Immunohistochemical staining was used to detect the expression of MMP-2、MMP-9 and TIMP-2 in 120 case of ovarian tumors.Gram’s straining was used to examine bacterial L-forms in these tissues.Results:The postive rates of MMP-2、MMP-9 and TIMP-2 were higher in malignant tumors than that in benign ones(P<0.005).The positive rates of MMP-2 and MMP-9 were higher in stage ⅠⅡ than that in stage Ⅲ,Ⅳ,and which increased with the grade (P<0.005).The expression of these two proteinases were higher in patients with lymph node involvement and ascites than those without that(P<0.05 to P<0.005).The positive expression of TIMP-2 was opposite to MMP-2 and MMP-9 (P<0.05 to P<0.005).The positive rate of bacterial L-forms was not higher in malignant tumors than that in benign ones(P>0.05),but it was increased with clinical stage and pathological grade (P<0.005).The expression of L-forms was higher in patients with lymph node involvement and ascites than those without that(P<0.005).Conclusions:In ovarian neoplasms,the abnormal expression of MMP-2、MMP-9、TIMP-2 and positive rate of bacterial L-forms have obvious relationship with clinical stage,pathological grade,lymph node involvement and ascites.Bacterial L-forms is maybe one of the inductive factors of the occurrence of tumor.MMP-2 、MMP-9 and bacterial L-forms perhaps have the function of positive cooperativity during the course of the occurrence of ovarian neoplasms.TIMP-2 perhaps accelerate the development of ovarian neoplasms.