肺癌组织survivi nmRNA的表达及其与预后的关系

Expression of survivin mRNA in lung cancer tissue and its relationship with prognosis

目的:探讨生存素(survivin)mRNA在肺癌组织中的表达与临床病理及其预后的关系。方法:应用逆转录聚合酶链反应(RT PCR)检测4 1例肺癌患者、9例良性肺疾病患者手术标本中的survivinmRNA表达,并随访2年,将其表达及表达强度与临床病理特征进行相关分析。结果:肺癌患者手术标本癌组织中survivinmRNA表达的阳性率(70 .7% )均高于癌旁正常组织(17.1% )和良性肺疾病患者组织survivnmRNA表达的阳性率(1/9) (P <0 .0 0 5 ) ;癌旁正常组织与良性肺疾病组织survivinmRNA表达的阳性率差异无显著性(P >0 .0 5 ) ;survivinmRNA在肺癌组织的表达及表达强度与年龄、性别、病理分型、病理分级、解剖部位分型及淋巴结和(或)不同肺叶及远处转移均无明显关系(P >0 .0 5 ) ,但与预后有明显关系(P =0 .0 0 18)。结论:肺癌组织检测survivinmRNA表达可以作为肺癌诊断的特异性分子标志物之一。

Objective:To assess the value of detection of survivin mRNA in lung cancer tissues.Methods:The specimens and the para-carcinomatous normal tissues of 41 patients with lung cancer and 9 patients with benign pulmonary diseases were collected.Reverse transcriptase polymerase chain reaction(RT-PCR) was performed to detect the survivin mRNA expression in the specimens.All the patients were followed-up for 2 years.The results were analyzed relatively.Results:The positive rate of survivin mRNA in the carcinomatous tissues(70.7%) was higher than that in the para-carcinomatous normal tissues(17.1%) and the benign pulmonary diseases tissues(1/9)(P<0.005).There was no significant difference between the para-carcinomatous normal tissues and the benign pulmonary diseases tissues(P> 0.05).survivin mRNA expression was not related to the age,sex,histological subtype,differentiation,pathologic grading or lymph node metastases.Conclusions:Expression of survivin mRNA in the lung cancer tissue can be used as a specific molecular marker for the diagnosis of lung cancer.It can act as an independent prognostic factor.