口腔鳞癌中VEGF表达和肿瘤相关巨噬细胞在血管生成中相互作用的初步研究

A Study on the Correlation between Vascular Endothelial Growth Factor and Tumor-associated Macrophagesin Tumour Angiogenesis of Human Oral Squamous-cell Carcinomas

目的:探讨口腔鳞癌中血管内皮生长因子(vascularendothelialgrowthfactorVEGF)和肿瘤相关巨噬细胞(tumor-associatedmacrophagesTAMs)在肿瘤血管生成中的关系。方法:采用免疫组化染色,光镜进行高倍视野下巨噬细胞、微血管计数、图像分析,以观察VEGF表达的强度,免疫组化双染观察巨噬细胞内VEGF的表达。结果:在口腔鳞癌中VEGF的表达与微血管计数(P<0.05,酌=0.412)及肿瘤相关巨噬细胞计数(P<0.05,酌=0.376)有关,免疫组化双染显示,TAMs内有VEGF的表达。结论:口腔鳞癌中与TAMs和VEGF表达有关,二者相互作用促进肿瘤的血管生成,参与肿瘤生长和转移。

Objective: To study the correlation between vascular endothelial growth factor and tumor-associated macrophages(TAMs) in angiogenesis of human oral squamous-cell carcinomas(OSCC).Methods: After immunohistochemical staining, light microscope was used for counting macrophages and microvessels, and automated image analysis quantification was used to detect VEGF expression. the double staining was also used to detect VEGF expression in TAMs. Results: VEGF expression show a significant positive connection with the counts of microvessels (P<0.05, r=0.412) and macrophages(P<0.05, r=0.376) in OSCC. TAMs express VEGF in OSCC. Conclusions: VEGF expression, macrophage infiltration and angiogenesis may represents a important synergistic inter-relation between tumor cells and stroma in the micro- environment of OSCC.