竞争间接ELISA检测谷物饲料中的玉米赤霉烯酮

Competitive Indirect ELISA with Anti-ZEN McAb for De-tecting the Mycotoxin Zearalenone in Corn , Wheat andFeedstuffs

应用抗玉米赤霉烯酮单克隆抗体（ＺＥＮ－ＭｃＡｂ）建立了竞争间接ＥＬＩＳＡ，用以检测玉米、小麦、饲料中的玉米赤霉烯酮（ＺＥＮ），最低检出量为０．０５ｎｇ／ｍＬ检测范围为０．０５～５００ｎｇ／ｍＬ。检测掺合ＺＥＮ（２５～５００ｎｇ／ｇ）的小麦、饲料、玉米样品，平均回收率，玉米为１０５．８％、小麦为９０％、饲料为１０３．９％；平均批内和批间变异系数，玉米为３．８％、５．８％，小麦为１２．７％、２８％，饲料为１５．２％、６．８％。对玉米、小麦、饲料自然样品检测，竞争间接ＥＬＩＳＡ的检出率高于薄层色谱（ＴＬＣ），检测程序简易快速。

A competitive indirect ELISA with anti-ZEN McAb for detecting themycotoxin zearalenone in corn, wheat and feedstuffs was developed. The minimum de-tectable amount was 0.05 ng/mL and the limits ranged from 0.05 ng to 500 ng permillilitre。 In the detection of the samples of corn,wheat and feedstuffs mixed with ZEN(2O-500 ng/g), the average rates of reclamation were 105.3%, 90%and 102.9%re-spectively. The mean intra-batch and inter-batch coefficients of variation were 3.8%and 5.8%in contaminated corn, 12.7%and 2.8%in contaminated wheat and 5.2%and 6.8%in contaminated feedstuffs, Using the developed competitive indirect ELISAto detect ZEN in natural samples of corn, wheat and feedstuffs , the detective rates washigher than those by thin-layer chromatography。 The assay procedure is simple, quickand easy to perform。