山羊黄体孕酮分泌的特点

The Secretion Pattern of Progesterone from the CorpusLuteum of the Goat

在山羊发情周期的第１０ｄ和第１７ｄ，分别给２只和１只山羊安置颈静脉导管和后腔静脉导管，以２０ｍｉｎ间隔连续采样２４ｈ。然后，将１只黄体中期羊卵巢摘除；给另１只黄体中期羊经静脉导管注射１５－甲基ＰＧＦ２ａ，每小时注射１次，每次２４０μｇ，连续７次。３只羊都继续采样。每次都同时采取颈静脉和后腔静脉血样，用ＲＩＡ法测定血浆孕酮（Ｐ４）水平。结果表明，山羊血浆Ｐ４水平没有昼夜节律；后腔静脉血浆Ｐ４水平有明显的波动，并高于颈静脉血浆Ｐ４水平（Ｐ＜０．０ｌ）；黄体溶解和卵巢摘除后，颈静脉和后腔静脉血浆的Ｐ４水平先后降到１ｎｇ／ｍＬ以下。由此证明，山羊Ｐ４是由黄体波动性地分泌的。

Two indwelling catheters were separately inserted ,in posterior caval veinand jugular vein of two goats at day 10 and other one at day l7 of the estrous cycle。 Blood samples were collected from the two catheters simultaneously everv 20 minutesfor 24 hours。 Thereafter,of the two goats at the middle corpus luteum phase,one was o-variectomized and another was administrated with 240 μg 15-meth PGF2α through theposterior caval vein catheter at a rhythm of once every hour for seven times。 Blood sam-ples were taken continually from all the three goats. P4 levels in the plasma sampleswere measured with RIA。 There was no circadian rhvthm in the P4levels。 Obvious fluc-tuation was observed in the P4 levels in the posterior caval vein but not in the jugularvein。 The P4 levels were significantly higher(P<0. 01 ) in the posterior caval vein thanthose in the jugular vein, Plasma P4levels fell below 1 ng/mL in both the posterior cavalvein and the jugular vein after ovariectomy , PGF2α-induced luteolysis and spontaneousluteolysis, The conclusion was that P4 was secreted undulantly from corpus luteum inthe goat。