牛卵母细胞电激活研究

Parthenogenetic Activation of Bovine Oocytes Matured In Vitro by Electric Stimulus

采用高场强单次脉冲（1600V/cm,80μs）及低场强3次脉冲（400V/cm,20μs,每次间隔20min）刺激体外成熟牛卵母细胞（成熟26h）,激活率分别为86.5%（167/193）和92.7%（101/109）,差异不显著（P>0.05）,其中多原核率分别为8.3%（16/193）和59.6%（65/109）,差异极显著（P<0.01）,但发育率差异不显著（20.6％和23.3％）。实验表明,电脉冲能显著提高牛成熟卵母细胞的激活率;多次电激会造成很大比例的多原核,但对卵母细胞的发育影响不大。

The object of this study was to determine the optimal conditions for parthenogenetic activation of in vitro matured bovine oocytes by electric stimulus. Oocytes were activated by A 1 pulse (1 600 v/cm, 80 us) or B 3 pulses (400 v/cm, 20 us, int. 20 min). It was found that there was no difference between the activation rates (AR) of A and B (86. 5% vs 92.7%, P>0.05),but more multipronuclei oocytes were produced from B (P<0.01). However, neither 1 pulse nor 3 pulses could improve the developmental rate (20.6% vs 23.3%,P>0.05). There was no difference between the AR of oocytes dipped statically in activation medium for 1 min and 5 min, either.