BV—SIVenv亚单位疫苗接种恒河猴的特异性CD8^＋Tc动态变化

The Kinetic Fluctuation of CytotoxiC CD8+T Cells Specif-ic for SIV in Macaea rhesus Inoculated with BV-SIV sub-unit Vaccine

用人ＥＢＶ转化恒河猴的自体Ｂ淋巴细胞，再以表达ＳＩＶ不同抗原成分的重组痘苗病毒ＶＡＣ－ＳＩＶｅｎｖ、ＶＡＣ－ＳＩＶｇａｇ感染，制备５１Ｃｒ释放试验的靶细胞。恒河猴于制备Ｂ淋巴细胞３０ｄ后，皮下接种ＢＶ－ＳＩＶｅｎｖ亚单位疫苗，每只动物１ｍＬ于强化免疫后的３０、６０、９０ｄ，从动物四肢内侧静脉采血，分离淋巴细胞，采用５１Ｃｒ释放试验检测动物体内的ＣＤ８＋Ｔｃ的动态变化。结果：１９只免疫恒河猴中，有３只于强化免疫后呈现明显的ＣＤ８＋Ｔｃ特异性细胞免疫反应，对靶细胞的杀伤活性在１６．７％～２０．２％之间，井可在体内维持３个月之久；在靶细胞数量不变，渐次增加效应细胞时，免疫细胞的ＣＤ８＋Ｔｃ的杀伤活性随数量增加而增强。从而证明该ＳＩＶ亚单位疫苗可使部分（１６％）免疫动物产生较好的特异性细胞免疫反应。

Autologous B-lymphocytes of Macaca rhesus transformed with EBV wereinfected with VAC-SIVgag or VAC-SIVenv that makes each product of SIV-gag or SIV-env,and prepared as targets for the 51Cr release assay。 The rhesus monkeys were sub-cutaneously inoculated with BV-SIVenv subunit vaccine at a dose of l mL per animal 30 davs later after successful preparation of the B-lymphocytes。Peripherial blood was col-lected from the veins of their limbs by 30,60 and 90 days after booster inoculation。 Lymphocytes were separated and used for evaluating the kinetic fluctuation of cytotoxic CD8+T cells in inoculated animals by 51Cr release assay。What we observed from this study was that 3 out of 19 animals inoculated with this vaccine showed apparent re-sponse of CD8+ T cells specific for SIV after booster,The specific killing activity of cy-totoxic CD8+ T cells to autologous targets ranged from 16.7%to 20.2%and retained about 3 months,The KIlling activities of cytotoxic CD8+ T cells derived from inoculated animals were strengthened according as the amount of targets were added,which sug-gesting that this SIV-subunit vaccine is able to induce strong cellular immune responses specific for SIV in immunized animals(16%)。