伊氏锥虫半－大规模体外培养初报

A Preliminary Report on Semilarge-scale Cultivation ofTrypanosoma evansi

按改良Ｂａｌｔｚ无细胞培养系统的培养方法，将盛有３００ｍＬ伊氏锥虫培养物、容量１７００ｍＬ（１１．０ｃｍ×２８．５ｃｍ）的培养瓶置于１０ｒ／ｍｉｎ的细胞培养旋转装置上，在３７℃培养箱中培养。在接种虫数为１．５×１０５～１０．０×１０５／ｍＬ时，其群体增倍时间为１２ｈ。接种虫数为１０×１０５／ｍＬ的培养物培养２４ｈ，每瓶收获约１．２×１０９锥虫，可满足一般实验的需要。

The 1700 mL culture flasks were individually inoculated with 300 mLTrypanosoma evansi cultures prepared by the method of modified Baltz's cell-free systemand revolved in a roller cell culture apparatus(10 r/min).The cultures were incubatedat 37℃. When the cell densities of inocula were 1.5× 105/mL,5. 0× 105/ mL or 10×105/ mL, the population doubling time was about 12h. The final cell density reached toabout 40×105/ mL in the last case and the yield of 1. 2×109 trypanosomes per flask maybe obtained after 24 hours' incubation, It was sufficient for the needs in routine experi-ments。