鸡传染性法氏囊病野毒株的分离鉴定及河北弱毒株培育的研究

Isolation and Identification of IBD Field Virus and Attenuation of Hebei Strains of IBDV

从河北省６个地区鸡传染性法氏囊病（ＩＢＤ）的鸡群中，分离到６株强毒，均能使易感鸡３６小时发病并死亡，发病率达６０～１００％，死亡率１０～７０％。人工感染鸡能见到与野外病例相同的病变。经血清学试验、鸡胚接种、电镜观察均证明，分离物为传染性法氏囊病毒（ＩＢＤＶ），其中有的毒株，毒力非常强。利用其中毒力最强的１株病毒，经鸡胚多次传代培养育成１株免疫原性好，免疫期长，免疫效果容易监测的弱毒株，经野外试验证明，该毒株是防治ＩＢＤ的一个很理想的毒株。

Isolated 6 strains of IBDV from sick chicken flock in Hebei Province. The viruses can infectsusceptible chickens in 36 hours. The morbidity was 60─100%and mortality was 10─70%, and the grosslesion were similar to the field cases. Serogical test,chicken embryo inoculation and electron microscopic ob-servation demonstrated that the isolation was a IBDV, some of them were high virulent strains. Using the vir-ulent strain of IBDV inoculated chicken embryo for many generations,We get a attenuted strains of IBDV.which is a good strain of immunity and long immunitic period and easy to inspect;It is a good vaccine to pro-tect IBD in the field.