禽传染性支气管炎病毒免疫原基因组的RT－PCR研究

Polymerase Chain Reaction Amplification of the S1 Glycoprotein Gene of Avian Infectious Bronchitis Virus

本文报道了用聚合酶链反应（ＰＣＲ）获取ＩＢｖＭ＿（４１）株和广东肾变株Ｄ＿（４１）免疫原（Ｓｌ）基因的研究情况，所用引物为ＩＢＶＢｅａｕｄｅｔｔｅ株Ｓ１基因两侧的对应序列，跨幅为１．７ｋｂ；ＤＮＡ模板为ＩＢＶＭ＿（４１）和Ｄ＿（４１）株经病毒ＲＮＡ提取后反转录而得；结果表明，２株病毒所获的ＰＣＲ产物与预期一致。

The polymerase chain reaction(PCR)was used to amplify the S1 glycoproteingene of avian infectious bronchitis virus(IBV).Two synthetic primers corresponding to seg-ments on both flanks of the spikeⅠ(S1) gene facilitated PCR amplification of a 1700-basesequence; Amplification was successful using complementary DNA(cDNA)created by re-verse transcription of IBV M41 strain and Guangdong nephrogenic strain D41 genomic RNA.The PCR product was the same as expected.