小麦抗白粉病基因聚合体DH材料的分子标记鉴定

Molecular Marker-Assisted Selection of DH Plants Conferring Genes Resistant to Powdery Mildew in Wheat (Triticum aestivum L. )

小麦白粉病是由Blumeriagraminisf sp tritici引起的世界性病害,利用分子标记辅助选择进行抗白粉病基因累加,可延长品种抗病性寿命,有利于提高育种效率。本研究利用Pm4b的STS PCR标记,Pm13和PmV的SCAR PCR标记,以及与Pm12共分离的同工酶标记(αAmy 1) ,对来自小麦与玉米杂交产生的双单倍体材料的7个株系和9个穗系的4 9个随机单株进行检测。分别筛选出含有Pm12 +Pm4b +PmV和Pm13+Pm4b +PmV 3个抗病基因的植株7个和1个,含有Pm12 +Pm4b、Pm4b +PmV、Pm12 +PmV和Pm13+Pm4b 2个抗病基因的植株3个、6个、2个和2个。利用小麦与玉米杂交方法创造多基因聚合体DH材料时,通过诱导愈伤组织,可产生更多不同基因类型的聚合体。

Powdery mildew, caused by Blumeria grami nis f. sp. tritici, is one of the most destructive wheat diseases world wide. Using molecular markers to pyramid more effective resistance genes is one way to sustain the resistance of wheat cultivars. The objective of this study was to identify the resistance gene pyramided in DH plants by molecular markers . A total of 49 DH plants derived from wheat × maize crosses and potentially c ontaining the multiple resistance genes were tested by the STS-PCR marker of Pm4b, SCAR-PCR marker of Pm13 & PmV, and the α-amylase isoenzyme marker of Pm12. The results showe d that seven plants conferred with Pm4b+Pm12+PmV, one plant with Pm4b+Pm13+PmV, three plants with Pm12+Pm4b , six plants with Pm4b+PmV, two plants with Pm12+PmV, two plants with Pm13+Pm4b, respe ctively (Table 1). When using wheat × maize crosses to produce resistance gene pyramided DH plants, it could produce more pyramided DH types through callus i nducing.