个体供血者丙型肝炎病毒基因亚型分析

Subgenotype Analysis of Hepatitis C Virus in paid Biood Donors

对血清抗-HCV阳性的65名个体供血者进行血清HCV-RNA检测,并作HCV基因分型,结果发现其中60例为HCV-RNA阳性,且均为HCV-Ⅱ型,提示本组个体供血者中以HCV-Ⅱ型为感染优势株。通过对HCV5＇-非编码区基因PCR为基础的核酸内切酶分型法和HCV核心区基因PCR直接分型法的比较发现,前者在敏感性及经济、简便等方面优于后者。

The detecting of HCV-RNA and genotyping of HCV in sera of 65 paid blood donors which are positive for anti-HCV were conducted,indicating that 60 of them appeared positive for HCV-RNA,and their genotype of HCV was classified as genotype- I . It was suggested that the genotype- I of HCV could be considered as the predominant infectious strain in this group of paid blood donors. It was found that the endonuclease digestion method of typing based on HCV 5' -noncoding region PCR was better compared with the direct method of typing based on HCV core region PCR in terms of its senstivity,economy and simplicity.