促红细胞生成素受体在慢性肾衰肾性贫血中的研究

The Study of Erythropoietin Receptor in Anemia of Chronic Renal Failure

目的:探讨促红细胞生成素受体(EPOR)在慢性肾功能衰竭肾性贫血中的表达及意义。方法:通过流式细胞仪技术检测30例慢性肾功能衰竭非透析患者、12例尿毒症透析患者、15例对照者(肾功能正常且无贫血)的外周血单个核细胞表面EPOR的表达。结果:重度肾性贫血患者外周血单个核细胞表面EPOR表达(7.06±5.48)%显著高于轻中度肾性贫血组(3.51±1.73)%(p<0.05)。慢性肾衰早期组外周血单个核细胞表面EPOR(5.36±3.15)%较对照组(2.12±1.75)%升高(p<0.01)。尿毒症透析组外周血单个核细胞表面EPOR(0.92±1.23)%较尿毒症非透析组(3.48±2.79)%显著降低(p<0.01)。慢性肾功能衰竭患者血清肌酐与血红蛋白(Hb)、红细胞压积(HCT)呈负相关。结论:重度肾性贫血时外周血EPOR表达异常升高,慢性肾衰时外周血EPOR表达较对照组升高,推测EPOR可能与肾性贫血的发生有关。

Objective: To study the use of erythropoietin receptor (EPOR) in anemia of chronic renal failure.Method:A total of 57 patients were enrolled in this study. 30 patients who were chronic renal failure (CRF) without dialysis. 12 patients who were treated with dialysis (10 patients with hemodialysis and 2 patients who were receiving continuous ambulatory peritoneal dialysis) .15 patients who have normal renal function (without anemia) were regarded as control group. The expressions of EPOR on peripheral blood mononuclear cells (PBMCs) were measured by flow cytometry in all patients.Result: The expressions of EPOR on PBMCs in patients with severe renal anemia(7.06±5.48)% were increased significantly compared with that in middle or slight renal anemia patients(3.51±1.73)%. The expression of EPOR on PBMCs in chronic renal insufficiency(CRI)(5.36±3.15)% was higher than that in control group(2.12±1.75)%. The expression of EPOR on PBMCs in uremic patients with dialysis(0.92±1.23)% was decreased significantly compared with that in uremic patients without dialysis(3.48±2.79)%. The serum creatinine level was relative to the level of Hb or HCT in CRF. Conclusion: The expressions of EPOR on PBMCs in patients with severe renal anemia were high unusually. The expressions of EPOR on PBMCs in CRI were higher than that in control group. EPOR may be related to renal anemia associated with CRF.