摘要
目的:探讨米非司酮对子宫内膜异位症细胞PTEN基因表达及凋亡的影响。方法:体外原代培养人异位子宫内膜细胞,应用四甲基偶氮噻唑兰(MTT)法测定子宫内膜异位症细胞体外增殖活性;应用流式细胞仪(FCM)检测在不同浓度米非司酮作用24h后,子宫内膜异位症细胞PTEN蛋白表达率、增殖率和凋亡率;应用吖啶橙荧光染色法和透射电镜技术研究子宫内膜异位症细胞的形态学变化。结果:米非司酮能够抑制子宫内膜异位症细胞的增殖且呈明显的剂量依赖性;AO荧光染色及透射电镜观察表明,经米非司酮培养的子宫内膜异位症细胞出现了典型的细胞凋亡的特征性变化;米非司酮能够使子宫内膜异位症细胞出现细胞周期阻滞,使其停滞于G1 期,降低S期细胞比例;PTEN蛋白的表达率明显增加,与对照组相比差异显著。结论:米非司酮能够明显抑制人异位子宫内膜细胞的体外增殖并促进其凋亡,其促凋亡作用与上调PTEN基因的表达有关。
Objective:To investigate the expression changes of PTEN gene and the apoptotic effect of ectopic endometrium cells that induced by mifepristone in vitro.Methods:Inhibition of proliferation cells measured by MTT assay.Morphological assessment was performed with fluorescence microscopy and electron microscopy.The cell cycle distribution and apoptosis were analyzed by flow cytometry.Results:Mifepristone could inhibite the proliferation of the ectopic endometrium cells in a concentration-dependent manner in vitro.Typical morphological characteristics of apoptosis induced by mifepristone could be observed by AO staining and transmission electron microscopy.Mifepristone could also block the proliferative cycle of ectopic endometrium cells at the G 1 stage,decrease the cell ratio of S stage.Compared with the control group,expression of PTEN protein significantly increased in mifepristone treated groups.Conclusion:Mifepristone can significantly inhibit the proliferation and induce the cell apoptosis of ectopic endometrium cells in dose-time dependent manner in vitro.Such effects are related to up-regulation of the expression of tumor suppressor gene PTEN in ectopoic endometrium cells.
出处
《现代妇产科进展》
CSCD
北大核心
2005年第2期138-141,F003,共5页
Progress in Obstetrics and Gynecology
