B19 VP2抗原真核表达载体的构建及免疫原性观察

Construction of eukaryotic expression vector pcDNA3/VP2 and its immunological effect

目的:探讨以B19VP2基因为靶基因构建的真核表达载体作为基因疫苗的免疫原性。方法:用PCR方法以pGEM1B19为模板扩增出B19VP2目的基因;将其重组入真核表达载体pcDNA3;将重组子pcDNA3VP2及空质粒分别用脂质体包裹接种家兔;ELISA方法检测家兔血清中B19VP2抗体产生情况。结果:成功构建了真核表达载体pcDNA3-VP2;其作为基因疫苗接种家兔后可产生滴度较高,持续时间较长的抗体。结论:B19VP2基因可作为B19病毒基因疫苗构建的靶基因。

Aim: To explore the immunogenicity of pcDNA3/VP2 as gene vaccine. Methods: B19 VP2 gene was amplified using PCR. B19 VP2 was recombined with pcDNA3 to form pcDNA3/VP2. pcDNA3/VP2 packed with liposome was inoculated into laboratory rabbit. B19 VP2 antibody was detected in rabbit's serum using ELISA.Results: Eukaryotic vector pcDNA3/VP2 was successfully constructed, which had better immunogenicity as gene vaccine and could stimulate rabbit to generate higher titre antibody. Conclusion: B19 VP2 gene can be used as target gene of B19 virus gene vaccine.