Rubisco及其活化酶定位于豌豆和蚕豆叶绿体中

Immunogold localization of Rubisco and its activase in chloroplasts of pea and broad leaves

运用免疫金标记电镜技术研究了豆科C3植物豌豆(PisumsativumL.)和蚕豆(ViciafabaL.)叶片中Rubisco及其活化酶(RCA)的细胞定位,结果表明:两种植物叶片解剖结构及叶绿体超微结构相似,叶肉细胞叶绿体具有发达的基粒片层,Rubisco和RCA免疫金标记颗粒主要分布于叶绿体的间质中,在基粒片层上很少,在表皮的气孔保卫细胞叶绿体内也有免疫金颗粒标记,在细胞质、液泡、线粒体等细胞器中无特异性标记。两种植物光合作用关键酶在叶绿体中定位的相似性,体现了C3植物在光合器结构与功能上具有共性。

Cellular localization of Rubisco and Rubisco activase(RCA) in pea (Pisum sativum L.) and broad (Vicia faba L.) leaves were investigated by immunogold-labeling electron microscopy. The results showed that the leaf anatomy and the chloroplast ultrastructure were extremely similar between pea and broad. Their mesophyll chloroplasts had well-developed grana and the stroma was densely labeled with immunogold particles for Rubisco and for RCA. The two photosynthetic enzymes were mainly located in chloroplasts of mesophyll and guard cell of stomatal apparatus. In contrast, there was no specific binding of gold particles detected in the cytoplasm, vacuole, mitochondria and other organelles. These results showed that the structure and function of chloroplasts are similar in C_3 plants.