三苯基锡化合物与牛血清白蛋白作用光谱

Interaction of Chlorotriphenyltin with BSA

研究了船体防污漆中主要防污成分———三苯基一氯化锡(TPTCl)与牛血清白蛋白(BSA)作用的光谱特征。结果表明,TPTCl与BSA的相互作用是双重的。既有TPTCl中锡离子与BSA的配位作用,使BSA分子中色氨酸和酪氨酸芳杂环残基裸露,又有TPTCl中苯环的疏水作用,使BSA分子发生收缩。随着反应时间不同,紫外光谱表现为先是明显的增色效应,后是增色效应略有减弱。且低浓度的TPTCl与BSA主要表现为锡离子与BSA的配位作用,高浓度的TPTCl与BSA主要表现为苯环的疏水作用。缓冲溶液的加入则有利于TPTCl与BSA的相互作用,但影响程度不同。增色效应顺序是KH2PO4-NaOH体系>Tris-HCl体系>HAc-NaAc体系>无缓冲液体系。

Chlorotriphenyltin(TPTCl) is one of the major components of ship fouling paints. The spectral (characteristics) of the interaction of TPTCl with Bovine Serum Albumin(BSA) were studied. The results show that BSA coordinates with the tin ion of the TPTCl, resulting in the exposure of aromatic heterocycle residues of tryptophane and tyrosine in BSA moledule, the (benzene) ring of TPTCl interacts with BSA through hydrophobic action, which leads to contraction of BSA molecules. The (obvious) hyperchromic effect was observed in the UV spectra at the beginning, which then weakened with the reaction time. In addition, the UV spectra and the CD spectra show that TPTCl (mainly) interacts with BSA through coordination when the TPTCl concentration is low, whereas the hydrophobic interaction dominates when the TPTCl concentration is high. The various buffer solutions added favors the interaction of TPTCl and BSA to different extent. The order(from strong to weak) of hyperchromic effects was KH_(2)PO_(4)-NaOH system, Tris-HCl system, HAc-NaAc system, and no buffer system.