摘要
Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds. The product structure was characterized by elemental analysis, IR, 1H NMR and 13C NMR. The effects of the reaction conditions on the enzyme-catalyzed reaction are discussed. The results show that the enzyme （activity） was greatly increased by adding SDS. The yield of the reaction was increased compared with that reported in the literature under the conditions of pH=5.6, temperature 30 ℃, Ca2+ 40mmol/L, （n（substrate ）∶）n（SDS）=（2∶1）, reaction time 8 h. The cost was lower. The melting point of the product was 69.5 ℃.
Diacetylenic glycerophosphatidylhydroxyethanol was synthesized from diacetylenic glycerophosphatidylcholine in the presence of phospholipase D from peanut seeds. The product structure was characterized by elemental analysis, IR, ^(1)H NMR and ^(13)C NMR. The effects of the reaction conditions on the enzyme-catalyzed reaction are discussed. The results show that the enzyme (activity) was greatly increased by adding SDS. The yield of the reaction was increased compared with that reported in the literature under the conditions of pH=5.6, temperature 30 ℃, Ca^(2+) 40mmol/L, (n(substrate )∶)n(SDS)=(2∶1), reaction time 8 h. The cost was lower. The melting point of the product was 69.5 ℃.
出处
《应用化学》
CAS
CSCD
北大核心
2005年第5期575-577,共3页
Chinese Journal of Applied Chemistry
基金
船舶工业国防科技应用基础研究基金资助项目(01J.1.47)
关键词
磷脂酶D
联乙炔基甘油磷脂酰胆碱
联乙炔基甘油磷脂酰乙二醇
酶催化反应
phospholipase D,diacetylenic glycerophosphatidylcholine,diacetylenic glycerophosphatidylhydroxyethanol,enzyme catalyzed reaction
