摘要
A highly selective and rapid anodic adsorptive voltammetric method was developed for the (determination) of ultra trace amount of ofloxacin at a carbon paste electrode(CPE). The ofloxacin adsorbed on the surface of the CPE in a 0.096 mol/L KHP-NaOH buffer solution(pH=5.6) could produce one oxidation peak at 0.92 V(vs.SCE) when scanning was performed from 0.4 to 1.4 V. The relationship between the peak current and the ofloxacin concentration was linear in the ranges of 5.5×10-9~5.5×10-8 mol/L(75 s accumulation) and 3.3×10-8~3.3×10-7 mol/L(30 s accumulation). The correlation coefficients were 0.996 and 0.998, respectively. Its detection limit was 9.0×10-10 mol/L(S/N=3) for 150 s accumulation. The (electrode) reaction mechanism of ofloxacin was investigated. The proposed method was applied to analyze trace ofloxacin in urine samples. The result was in good agreement with that obtained by spectrophotometry (determination).
A highly selective and rapid anodic adsorptive voltammetric method was developed for the (determination) of ultra trace amount of ofloxacin at a carbon paste electrode(CPE). The ofloxacin adsorbed on the surface of the CPE in a 0.096 mol/L KHP-NaOH buffer solution(pH=5.6) could produce one oxidation peak at 0.92 V(vs.SCE) when scanning was performed from 0.4 to 1.4 V. The relationship between the peak current and the ofloxacin concentration was linear in the ranges of 5.5×10^(-9)~5.5×10^(-8) mol/L(75 s accumulation) and 3.3×10^(-8)~3.3×10^(-7) mol/L(30 s accumulation). The correlation coefficients were 0.996 and 0.998, respectively. Its detection limit was 9.0×10^(-10) mol/L(S/N=3) for 150 s accumulation. The (electrode) reaction mechanism of ofloxacin was investigated. The proposed method was applied to analyze trace ofloxacin in urine samples. The result was in good agreement with that obtained by spectrophotometry (determination).
出处
《应用化学》
CAS
CSCD
北大核心
2005年第5期578-580,共3页
Chinese Journal of Applied Chemistry
基金
中国科学院长春应用化学研究所电分析化学国家重点实验室基金资助课题
关键词
氧氟沙星
阳极吸附伏安法
碳糊电极
ofloxacin,anodic adsorptive voltammetry,carbon paste electrode