摘要
研究了不同的培养时间、翻曲及NaCl对本课题组分离得到的毛霉的蛋白酶活力的影响,探讨了不同的毛霉蛋白酶提取方法,并对比了福林酚法测定蛋白酶活力时的最佳吸收波长。结果表明:该株毛霉麸曲固态培养72h时蛋白酶活力最高;培养24h后翻曲一次能显著提高酶活力;用盐水麸曲制曲培养时,用蒸馏水提取可获得较高活力的复合蛋白酶;测定蛋白酶活力时,最佳吸收波长为690nm。
This paper dealt with the effects of culture time, culture-disturbing, and NaCl on protease activity of the mucor extracted. Several methods for the extraction of mucor protease with optimal absorb wavelength for detection of protease activity were also studied. The results were that: high composition protease activity could be obtained through 72h solid-medium cultivation, but one culture-farmed-over after 24h cultivation, extracted with distilled water in using NaCl in the culture would yield higher activity. The optimal absorb wavelength was 690nm.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第5期44-47,共4页
Food Science
关键词
蛋白酶
毛霉
培养时间
翻曲
提取
Protease
mucor
culture time
culture-farmed-over
separation
