海藻糖合成酶的分离纯化及部分酶学性质研究

Studies on Purification and Properties of Trehalose Synthase Enzyme Isolated from Arthrobacter nicotinovorus

食尼古丁节杆菌JNU-1(ArthrobacternicotinovorusJNU-1)菌株所产胞内海藻糖合成酶,该酶能转化麦芽糖合成海藻糖。将食尼古丁节杆菌JNU-1进行大量的培养,富集发酵菌丝体,经超声破碎、浸提、离心后获得粗酶液,粗酶液经硫酸铵分级沉淀后,收集相应的活性部分,用葡聚糖凝胶进一步纯化,通过电泳来测定海藻糖合成酶的纯度和分子量。

Arthrobacter nicotinovorus (JNU-1)can produce trehalose synthase enzyme. Study finds that trehalose synthase enzyme is a kind of enzyme in the cell. After substantive cultivation of bacterium JNU-1 and enrichment of culture mycelium, the cells harvested were disrupted by ultrasonic, followed by leach and centrifugation to obtain crude enzyme solution. The crude enzyme was fractionated with ammonium sulfate and the active fractions were collected, dialyzed, concentrated and successively purified by column chromatography packing Sephadex G-100. The purity and molecular weight of the enzyme were determined by SDS polyacrylamide gel electrophoresis.