加杨雄花序提取物体外抗氧化及对DNA损伤的保护作用研究

Effects of the Extract of Populus canadensis Moench. Male Anthotaxy on Antioxidation and Prevention of DNA Damage Caused by Hydroxyl Radical in vitro

利用比色法、化学发光法,研究了加杨雄花序提取物体在体外对O2-·,·OH,H2O2活性氧自由基的清除作用,以及通过Cu2+-Phen-H2O2-Vit.C-DNA发光体系研究加杨雄花序提取物对·OH引起的DNA氧化损伤的保护作用。实验结果表明加杨雄花序提取物对O2-·,·OH,H2O2自由基均有明显的清除作用,其50%抑制浓度(IC50)分别为0.69、1.73、0.23mg/ml,并能显著抑制·OH对DNA氧化损伤。

The effects of the extract of male anthotaxy of Populus canadensis Moench. on removal of active oxygen species in some modified chemical systems were investigated by chemiluminescence and spectrophotometry. The inhibition of the damage of DNA chain induced by hydroxyl radical by the extract from male anthotaxy was observed by chemiluminescence. The results showed that the extract could efficiently remove O2-·,· OH, H 2 O 2 and the 50% inhibition concentrations (IC 50 ) were 0. 69,1.73, 0.23mg/ml, respectively. The extract also protected DNA chains from being damaged by hydroxyl radical.