两相分配生物反应器——浊点系统在生物转化中的应用

Two-phase Partitioning Bioreactor, Application of Cloud Point System in Bioconversion

利用两相分配生物反应器可以控制底物由非水相向水相释放,增加底物的溶解度和解除底物对微生物的抑制,保护产物降解,降低下游分离费用;论述了两相分配生物反应器的基本原理和发展概况,并以胆固醇边链切除生物转化为例,介绍了新开发的浊点系统两相分配生物反应器的巨大潜力。

The two-phase partitioning bioreactor is usually applied in many bioprocesses for enhancing the productivity. The controlled substrate delivery from the non-aqueous phase to aqueous phase opens a new area of application of this strategy for enhancing the substrate solubility and eliminating the substrate inhibition of bioprocesses, such as bioconversion, biodegradation, etc. The extraction product from bioreaction phase has advantages over conventional media, such as removing the product inhibition on the microorganism, protecting the product form further degradation and cutting down the effluent treatment costs as a result of the use of a more concentrated feedstock. This article examines recent research on methods of the two-phase partitioning bioreactor. The practical potential of cloud point system as a novel two-phase partitioning bioreactor is illustrated by reference to a particular bioconversion, namely the side-chain cleavage of cholesterol to produce a pharmaceutical intermediate androsta-diene-dione (ADD).