水窖水与大田烟草病毒病的关系

Relationship between Tobacco Viruses in Vault Water and Incidence of Tobacco Virus Diseases in Field

为查清水窖水体是否带有烟草病毒及水窖水带病毒与烟草病毒病的关系,研究了田边水窖水样和用其水在烤烟返苗期和现蕾期灌溉烟田后烟株发病情况及其病叶样品的病毒种类。结果表明:水窖水带TMV;现蕾期烟株发病率为1 8%～16 8%,平均发病率为8 6%;病叶的TMV、CMV检出率分别为45 8%和27 8%,PVY和TEV检出率为27 8%。

In order to investigate whether the viruses affecting tobacco were present in vault water and their relation with Tobacco Mosaic Virus (TMV), the water was sampled and tested with Indirect-ELISA and inoculated into Nicotiana cordifolia at seedling restitution and budding stages of flue-cured tobacco in Qujing of Yunnan. After (irrigated) with the water, field virus disease incidence was investigated and diseased leaves were sampled randomly and the viruses identified. The results showed that there was TMV in vault water; at budding stage, the average (incidence) of virus disease was 8.6%, ranging from 1.8% to 16.8%; the positive ratio of TMV, CMV, PVY and TEV in infected leaves were 45.8%, 27.8%, 27.8% and 27.8%, respectively.