抗原肽运载体TAP2基因与乙型肝炎病毒感染预后的相关性研究

Genetic polymorphisms of TAP2 in patients with hepatitis B virus infection

目的 研究上海地区中国人群抗原肽运载体(transporter associated with antigen processing, TAP)基因与HBV感染以及慢性HBV感染和慢性肝炎并发肝硬化之间的相关性。方法 收集临床病例,检测乙型肝炎病毒标志,参照“病毒性肝炎防治方案2000”,选取101例乙型肝炎病毒感染者,21例HBV感染康复者及113例正常对照,进行TAP2 等位基因分型。对数据分组进行统计分析。结果 慢性乙型肝炎组的TAP2 *0201基因频率显著低于感染恢复组(χ2 =4.95, P<0.03);肝硬化组的TAP2 *0101基因频率显著高于正常对照组(χ2 =5.72, P<0.02);在慢性乙型肝炎并发肝硬化组中DR4与TAP2 *0101等位基因间存在显著的连锁不平衡(△=0.026,P<0.05)。结论 TAP2 *0101等位基因型携带者易感慢性乙型肝炎和乙型肝炎并发肝硬化,而TAP2 *0201则表现出对慢性乙型肝炎的抗性。两者均与HBV感染的预后似有一定相关。

Objective To investigate the polymorphisms of TAP2 gene in Chinese patients with HBV infection, chronic hepatitis B, hepatocirrhosis and individuals recovered from HBV infection. Methods The investigation covers 101 chronic hepatitis B patients and 21 subjects recovered from HBV infection. A healthy control panel of 113 subjects was collected from Shanghai Blood Center. All of the specimens subjected to the definition of the Blue Print of Prevention and Cure of Virosis Hepatitis-2000. TAP2 gene genotypes were determined by means of PCR-RFLP and ARMS-PCR, and their frequencies were compared. Results Frequency of TAP2*0201 in chronic hepatitis B was significantly lower than that in the recovered (χ2=4.95, P<0.03). Frequency of TAP2*0101 in hepatocirrhosis was significantly higher than that in healthy control(χ2=5.72, P<0.02); The TAP2*0101 allele was a strong linkage disequilibrium with HLA-DR4 in hepatocirrhosis in this study (△=0.026, P<0.05). Conclusion These results suggested that TAP2*0101 allele might be associated with susceptibility of chronic hepatitis B and hepatocirrhosis in Shanghai patients, however TAP2*0201 revealed to be resistant to chronic hepatitis B. Both of the two alleles were closely associated with the prognosis of HBV infection.