石家庄地区人群中PPARγ_2基因突变与瘦素分泌和肥胖症关系的初步研究

The relationship among peroxisome proliferator-activated receptor γ_2( PPARγ_2) gene mutation and leptin secretion and adiposity in Shijiazhuang area.

目的 旨在研究过氧化物酶增殖物活化受体γ2 (PPARγ2 )基因Pro12Ala多态性与Leptin分泌和肥胖症的关系。方法 选择石家庄地区汉族2 0 8例,非肥胖和肥胖个体人,应用聚合酶链式反应-限制性片断长度多态性分析(PCR-RFLP) ,检测PPARγ2 基因突变。酶联免疫法(ELISA)测定血清瘦素(Leptin)浓度。结果 研究对象中非肥胖与肥胖个体血清瘦素浓度有显著性差别,非肥胖与肥胖组中均存在PPARγ2 基因Plo12和Ala12变异,但肥胖组中PPARγ2 基因Plo12和Ala12变异频率明显增高。结论 肥胖个体中PPARγ2 基因有Ala12突变是导致血清瘦素分泌增高、肥胖症发生有密切关系的遗传因素之一。

Objective:To study the relationship among PPARγ 2 gene Pro12Ala polymorphism and leptin secretion and adiposity. Methods:208 cases were chose in Shijiazhuang area including adiposity group and control group. PPARγ 2 gene mutation was examined using PCR- RFLP, and serum leptin concentration was examined using ELISA.Results:The serum leptin concentrations of the adiposity group were significantly different from that of the control group. The two groups both had the PPARγ 2 gene mutation (Pro12Ala), but the frequency of the gene variation of the adiposity group was higher than that of the control group. Conclusion:The Ala12 variation in PPARγ 2 gene of adiposity is one of the inherited factors, which speed the serum leptin's secretion and have germane relationships with adiposity.