骨骼肌卫星细胞移植后心肌梗死区血管内皮因子表达及血管新生作用

The vascular endothelial growth factor expression and vascular regeneration in infarcted myocardium by skeletal muscle satellite cells

目的:研究骨骼肌卫星细胞移植后心肌梗死(MI)区血管内皮因子(VEGF)表达及血管新生作用。方法:成年Louis近交系大鼠,结扎前降支建立急性MI模型,将骨骼肌卫星细胞移植到梗死区,2W后应用免疫组化方法鉴定梗死区VEGF的表达及新生血管密度。结果:骨骼肌卫星细胞在梗死区增殖、分化良好,梗死区VEGF表达移植组明显高于对照组(P<0.05),新生血管密度移植组也高于对照组(P<0.05)。结论:移植区卫星细胞可以分泌生长因子,促进血管形成,改善梗死区细胞的生存环境。

Objective:To study the vascular endothelial growth factor expression and vascular regeneration in infarcted myocardium by skeletal muscle satellite cells. Method: The skeletal muscle satellite cells were planted into the infarcted myocardium by ligated left anterior artery in Louis Inbrad Strain rat. Specimens were got for identifying the expression of VEGF and the density of vascular by immunochemical method at two weeks after implantation. Result:The proliferation and differentiation of the skeletal muscle satellite cell were very well. The expression of VEGF was higher in the implanted group than that in the control group (P< 0.01). The vascular density in the implanted group was also higher than that in the control group (P< 0.05). Conclusion:The implanted satellite cell can excrete growth factor which can promote vascular producing and improve survival environment of cells in infarcted myocardium.