摘要
用38只大耳白兔随机分成5组。Ⅰ组:正常对照组;Ⅱ组:单纯缺血组;Ⅲ组:缺血再灌注60min组;Ⅳ组:甘露醇合剂治疗组;Ⅴ组:盐水治疗对照组。结果:Ⅳ组肺组织丙二醛含量无明显增高,与Ⅱ组、Ⅲ组及Ⅴ组相比相差非常显著(P<0.01)。血气分析、WBC计数、WBC粘附率、肺组织D/W重比等指标,Ⅳ组与Ⅲ组及Ⅴ组相比均有显著差异(P<0.05~0.01)。Ⅳ组肺组织学仅见微血管轻度充血、少量PMN聚集。实验结果揭示:甘露醇、抗坏血酸对下肢缺血-再灌注后所致急性肺损伤有较好的防治作用。
This study indicatcd iscbemia reperfusion oflower limbs might induce acute pulmonary
injuty,Wealmed at Investigating the protective function ofmannitol and ascorbic acid (oxygen
free raical scav-enger) by using pulmonary function and histologicaland ultrastructural indexes
in experimental anim alsin the hypoxic area of plateau (3,700 meters abovesea level).The
animals were radomly d ivided intofive groups:group I:normal controls; group Ⅱ:pure
ischemia;group Ⅲ:ischemia reperfusion for 60minutes;group Ⅳ:treated with mixture of
mannitol;group Ⅴ:controls with the administration ofsalinc,In group Ⅳ,the animals had
ischemia in theirlower limbs for four hours and intravenous injectionof mixture of mannitol was
given at the dosage of5ml/kg,30 and five minutes before the restorationof blood now.and five
and 30 minutes after the res-toration of blood flow,In group Ⅴ,normal salinewas injected at the
same dosage and at the same timeas mentioned in group Ⅳ。The results were asfollows:the
content of MDA did not appear to havea marked increase,which indicated the existence of a
significant difference as compared with groups Ⅱ,Ⅲand V(P<(0.01); blood gas analysis, WBC
count,vvBC adhesive rate and pulmonary tissue D/W ingroup Ⅳ were shQ wn to be significantly
different ascompared with group Ⅲ and group V(P<0.05~0.01);bistologically and
ultrastructurally,onlymildmicrovascular congestion and aggregation of a smallamount of
polymorphonuclears could be seen in thelungs in gtoup Ⅳ. It is suggested that mannitol
andascorbic acid appear to have a favorable preventiveant therapeutic effects on acute
pulmonary injury af-ter ischemia reperfusion of the lower limbs.Hence,early use of mannitol and
ascorbic acid in patientswith ischemie limbs is recommended.
出处
《中国危重病急救医学》
CAS
CSCD
1994年第1期7-9,共3页
Chinese Critical Care Medicine
关键词
甘露醇
肢体缺血
再灌注
肺损伤
manitol
ischemia
repermsion
pulmo-nary injury