摘要
将２１只大鼠随机分成肿瘤坏死因子作用２４ｈ组、７２ｈ组和生理盐水对照组。实验组用重组人的肿瘤坏死因子α型（ｒｈ－ＴＮＦ－α）×１０￣５Ｕ·ｋｇ￣（－１）／ｄ持续静脉泵入，分别于２４ｈ．７２ｈ活杀，观察血液及心、肝、肺、肾、肠组织ＴＮＦ水平；过氧化物歧化酶（ＳＯＤ）活性；丙二醛及血浆内毒素含量；组织超弱发光和白细胞吞噬发光、血气、肝肾功能及血培养情况。结果：实验２４ｈ组血浆ＴＮＦ显著升高，各脏器功能无明显受损，血培养及内毒素测定阴性、白细胞吞噬功能无变化；但ＴＮＦ可使血浆及组织氧自由基和脂质过氧化产物增加，全血ＳＯＤ活性下降。
21 rats wete randomly divided into the experi-mental groupⅠ
[infusion of recombinant humantumor necrosis factor-a(rh-TNF-a)for 24 hrs],group Ⅱ(infusion of
rh-TNF-a for 72 hrs)and thecontrol group (infusion of normal saline).The ani-mals in the
experimental group A and group B werekilled 24 hrs and 72 hrs after infusion of
rh-TNA-arespectively,TNF concentrations,activities ofsuperoxide dismutase(SOD)and MDA in the
blood,hearts,livers,lungs,kidneys and intestinal tissue,lev-els of plasma endotoxin and blood
gases were deter-mined; the hepatic and renal functions were tested;blood cultures were
obtained:the ultra-weakchemiluminescence of tissue and the luminol-edped-dent
chemiluminascence of PMN were mea-sured.Marked increase of plasma TNF was found inthe
experimental group 24 hrs after infusion ofth-TNp-a.but no significant damage was seen inthe
vital organs. Blood cultures and endotoxin werenegative.Phagocytosis of PMN was not shown
tohave any change,However,TNF might be the causeof the increase of oxygen-derived free
radicals in theplasma and tissue,and the cause of the decreased ac-tivities of SOD.
出处
《中国危重病急救医学》
CAS
CSCD
1994年第2期75-77,共3页
Chinese Critical Care Medicine
基金
全军青年基金
