膀胱移行细胞癌组织中黑色素瘤抗原基因及蛋白的表达

Expression of melanoma antigen gene in urinary transitional cell carcinomas

目的探讨膀胱移行细胞癌(TCC)中黑色素瘤抗原(MAGE)基因mRNA的表达及其临床意义。方法采用逆转录聚合酶链反应(RT PCR)技术检测3个膀胱TCC细胞株和20例膀胱TCC患者癌组织(新鲜标本,T1期7例,T2期5例,T3期6例,T4期2例;G11例,G211例,G38例)MAGE A1,A2,A3,A4mRNA表达,免疫组化法检测105例膀胱TCC患者癌组织(石蜡标本,T1期35例,T2期12例,T3期26例,T4期13例,另19例无法确定分期;G113例,G244例,G348例)MAGE A4蛋白的表达。结果3个膀胱TCC细胞株均有MAGE基因mRNA的表达;20例膀胱TCC新鲜标本组织MAGEmRNA阳性:A112例(60%),A216例(80%),A311例(55%),A418例(90%),A1～A4均阳性8例(40%)。105例膀胱TCC石蜡标本组织中MAGE A4蛋白阳性53例(50%),高分级膀胱TCC组织中强表达(++或+++)率为27%(13/48),显著高于低分级的4%(2/57)(F=12.00,P<0.01),高分期膀胱TCC组织中强表达率为27%(14/51),显著高于低分期的0%(0/35)(F=11.48,P<0.01)。结论MAGE基因在膀胱TCC中有较高表达,分级或分期高的膀胱TCC中MACE A4蛋白明显强表达。

Objective To evaluate the significance of melanoma antigen(MAGE)gene expression in bladder transitional cell carcinoma (TCC). Methods MAGE-A1,A2,A3,A4 mRNA expression was detected by reverse transcription polymerase chain reaction ( RT-PCR ) in 3 clusters bladder TCC cells and 20 samples of bladder TCC patients(T_1 7 samples,T_2 5 samples,T_3 6 samples,T_4 2 samples,G_1 1 samples,G_2 11 samples,G_3 8 samples). MAGE-A4 protein was detected by immunohistochemistry in 105 samples of bladder TCC patients(T_1 35 samples,T_2 12 samples,T_3 26 samples,T_4 13 samples,G_1 13 samples,G_2 44 samples,G_3 48 samples). Results Three clusters bladder TCC cells had MAGE gene mRNA expression. In detection of MAGE mRNA of 20 samples of bladder TCC patients,12 samples (60%) expressed MAGE-A1, 16 samples (80%) expressed MAGE-A2, 11 samples (55%) expressed MAGE-A3, 18 samples (90%) expressed MAGE-A4, 8 samples (40%) expressed all of MAGE-A1—A4. In 105 bladder TCC samples, 53 samples (50%) expressed MAGE-A4 protein. Strong expession (++ or +++) was significant higher in higher grade(13 samples/48 samples)or stage(14 samples/51 samples)than in lower grade(2 samples/57 samples)or stage(0 samples/35 samples). Conclusion MAGE gene highly expresses in bladder TCC. Bladder TCC of high grade or high stage has higher MAGE-A4 protein strong expression.