盐酸小檗碱对实验性大鼠胃癌前病变细胞凋亡及生存素基因表达的影响

EFFECTS OF BERBERINE ON APOPTOSIS AND SURVIVIN GENE IN EXPERIMENTALLY INDUCED DYSPHASIA OF GASTRIC EPITHELIA CELL

目的 :研究盐酸小檗碱对实验性大鼠胃癌前病变细胞凋亡及survivin(生存素 )基因表达水平的影响。方法 :采用N 甲基 N 亚硝基胍 (MNNG)饮水法建立大鼠胃癌前病变模型 ,用盐酸小檗碱预防和小、中、大三种剂量干预治疗 ,光镜观察胃癌前病变的发生率 ,TUNEL(末端DNA标记法 )检测凋亡率 (AI) ,免疫组化法检测细胞中survivin基因表达水平。结果 :经盐酸小檗碱干预后 ,大鼠癌前病变的发生率明显降低 ,与模型组比较具有显著性差异 (P <0 .0 5 ) ,细胞凋亡率显著提高 ,三种剂量组与模型组比较具有显著性差异 (P <0 .0 1) ,并伴有survivin基因表达水平降低 ,其中以大剂量组尤为显著 ,与模型组比较具有显著性差异 (P <0 .0 1)。结论 :盐酸小檗碱能预防和治疗实验性大鼠胃癌前病变 ,其机制与提高细胞凋亡率和下调survivin基因表达有关。

Objective:to study the effect of berberine on the apoptosis and protein levels of survivin in experimentally induced dysphasia of gastric epithelia cells. Methods:first, make the apoptosis models of gastric cancer by using MNNG method. And apply the prevention, small, medium and large dosages of berberine to experimental models to interfere with normal treatment. Then observe the ratio of occurrence of gastric epithelia under an electro-microscope. Furthermore,measure the ratio of apoptosis by using TUNEL as well as the express of survivin protein by the method of immunohistochemistry. Results:berberine can enhance apoptosis index (AI) in experimentally induced dysphasia of gastric epithelia cells. After the berberine interference treatment to experimental groups, the ratio of gastric epithelia was obviously decreased comparing with model group(P<0.05).There was significantly difference between experimental groups and model group(P<0.01). There were remarkable difference between large dose group and model group(P<0.01).Conclusion:berberine can prevent and treat the epithelia of gastric cancer on experimental rats. Its mechanism is related to increased apoptosis ratio and decreased surviving gene.