地塞米松对肺纤维化大鼠肺组织超微结构的影响

Effects of dexameathone on pulmonary fibrosis induced by bleomycin and oxygen-derived free radicals in rats

目的:探讨地塞米松对肺纤维化大鼠肺组织氧自由基和超微结构的影响。方法:SD大鼠经气管内灌注博莱霉素诱导肺纤维化,随后每日注射地塞米松3.33mg·kg- 1干预。于注药后d 2 9处死。通过HE染色和透射电镜观察显微结构的变化,并通过测定肺组织超氧化物歧化酶(SOD )、丙二醛(MDA)来评价干预效果。结果:地塞米松组与博莱霉素组相比,肺组织SOD下降,MDA升高,肺纤维化程度减轻。结论:地塞米松能减轻大鼠肺纤维化,部分机制与抗氧化有关。

AIM: To study the ultrastructure of dexamethaone on the bleomycin induced pulmonary fibrosis. METHODS: Thirty Sprague-Dawley (SD) rats were randomly divided into three groups (n=10 in each): control group, bleomycin (BLM) group and dexamethasone (DXM) group. After 28 days, the MDA and SOD of lung were measured and the histology was detected. RESULTS: In DXM group, the concentration of SOD was lower than that in the BLM group (P< 0.01 ), and the degree of fibrosis was more mitigative than that in the BLM group by microscope and ultrastructure. CONCLUSION: Dexamethasone can reduce the damage of oxygen-derived free radicals of bleomycin. Treatment of dexamethasone may be useful in inhibiting pulmonary fibrosis.