摘要
目的探索检测胃癌患者腹膜微转移的方法。方法收集67例胃癌和9例胃良性病变患者腹腔冲洗液以及临床病理资料,分别采用巢式RT PCR检测腹腔冲洗液中角蛋白19(CK19)mRNA、癌胚抗原(CEA)mRNA、角蛋白20(CK20)mRNA表达;采用时间分辨荧光(TRF)检测腹腔冲洗液上清液中CEA蛋白(P CEA)含量;采用H E染色进行腹腔冲洗液细胞学(PLC)检查。结果患者腹腔冲洗液中CK19mRNA和CEAmRNA的阳性检出率分别为62.7%和52.2%,明显高于P CEA(35.8%)、CK20mRNA(34.3%)和PLC(31.3%)(P<0.01),且阳性率随肿瘤浸润深度、TNM分期及腹膜受累程度增加而增加。结论巢式RT PCR联合检测胃癌患者腹腔冲洗液中CK19及CEA基因,可提高检测腹腔游离肿瘤细胞的灵敏性,适用于预测胃癌患者腹膜微转移。
Objective:To explore a method for predicting peritoneal micrometastasis in gastric carcinoma patients. Methods: Peritoneal washings of 67 patients with gastric carcinoma and 9 patients with benign gastric diseases were collected to detect the mRNA level of CK19,CEA and CK20 by nest reverse transcription-polymerase chain reaction (RT-PCR). The protein level of CEA(P-CEA) was detected by time resolved fluoroimmunoassay(TRF). The peritoneal lavage cytology examinations (PLC) were carried out by H-E staining.Results: The positive rates of CK19 mRNA, CEA mRNA and CK20 mRNA in peritoneal washings were 62.7%, 52.2% and 34.3%,respectively. The positive rates of CK19 mRNA and CEA mRNA were significantly higher than those of P-CEA (35.8%) and PLC (31.3%). The positive rates of CK19 mRNA and CEA mRNA were concurrently increased with the depth of tumor invasion, TNM staging and degree of peritoneum involvement. Conclusion: Detecting CK19 mRNA and CEA mRNA in peritoneal washings by nest RT-PCR can improve the sensitivity of free tumor cell detection in peritoneal washings,and may be used to predict peritoneal micrometastasis of gastric carcinoma.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第5期492-495,共4页
Academic Journal of Second Military Medical University
基金
上海市卫生局科研基金面上项目(024011).
