CMV43^(CT)转基因小鼠心血管发育的形态学研究

Morphological Study of Heart Development in CMV43^(CT) Transgenic Mice

目的 研究CMV4 3CT转基因小鼠心血管发育,探讨其用作先天性心脏畸形动物模型的可能性。方法选用胚胎期(ED) 12 .5～17.5d的CMV4 3CT胎鼠,经HE染色,观察心脏的形态学变化。对生后不久死亡的CMV4 3CT纯合转基因小鼠在显微镜下进行大体解剖,观察心脏外形的变化并切片行HE染色。通过PCR方法鉴定基因型,普通C5 7BL6 /SJ小鼠作为对照组。结果 所有对照组胎鼠和CMV4 3CT杂合胎鼠均未见心脏畸形。见6 2只纯合胎鼠中有14只心脏畸形,占2 2 .6 % ,主要为圆锥干发育畸形和室间隔缺损。其他畸形包括:心管环化异常及心室肌发育不良。12只生后不久死亡的CMV4 3CT纯合转基因小鼠大体解剖后发现均有室间隔裂和流出道囊等心脏畸形。切片染色发现左、右室流出道梗阻、房间隔缺损、室间隔缺损、心肌异常肥厚及心肌发育不良等畸形。结论 CMV4 3CT转基因小鼠存在以圆锥干发育异常为主的心血管畸形,可作为研究先天性心脏病的动物模型。

Purpose In this project we studied the mo rp hological features of the developing hearts in the CMV43 CT transgenic mice in order to evaluate the possibility and feasibility of being a new animal mode l of congenital heart malformations. Methods PCR analysis was carried out to identify the ge notypes of CMV43 CT transgenic mice.The embryos of ED12.5～17.5 and the new born mice died at birth were collected and embedded.Sections with HE staining we re analyzed to display the morphologic stuctures of the hearts in CMV43 CT transgenic mice.C57BL6/SJ mice were used as control. Results In 62 homozygous CMV43 CT fetus,14(22.6%) had heart defects including conotruncal defects (64.3%),ventricular septal defec t (50%),abnormal heart looping and others.Twelve homozygotes died at birth had b een found the interventricular cleft and the outpounching of the outflow tract.A nd we also found defects as obstructive lesions of the outlet of right/left vent ricle,ventricular septal defect and aboormal morphology of myocardium in these n ewborns. Conclusions The CMV43 CT transgenic mouse is a new animal model for studies of congenital heart malformations.