摘要
从300条随机引物中筛选出能稳定扩增的26个引物,对黄瓜育成品种"春玉"等21个实验材料进行扩增,在扩增出的173条谱带中,多态性带有80条,比例为46.24%。"春玉"在用引物E13扩增时有特异缺失条带,大小为400bp,可作为特征性指纹图谱,为其产权保护提供分子依据。利用各材料的DNA指纹可将不同参试材料鉴别出来。同时利用MEGA软件进行UPGMA聚类分析,将参试材料在相似系数0.706处分为4个组群。
26 primers that could be stably amplified were selected from 300 random primers and were used to amplify the DNA of 21 cucumber accessions including our newly breed cultivar 'Chunyu'.Eighty bands showed polymorphism among the 173 amplified bands.The percentage of polymorphism was 46.24%.The unique 400 bp missing band with E13 primer in cultivar 'Chunyu' could be used as fingerprint for the protection of its intellectual property.All the accessions used in this study could be identified based on the DNA fingerprints obtained.The accessions were classified into four groups at similarity coefficient 0.706 in UPGMA cluster analysis using MEGA software.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第5期876-880,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家高技术研究发展计划专项经费(2002AA241251
2004AA241120)
国家自然科学基金(30170644)
上海市科技兴农重点攻关项目资助(2001-3-3)
关键词
黄瓜
RAPD
DNA指纹
Cucumis sativus L.
RAPD
DNA fingerprints
