摘要
采用酶解的方法对大麦中β葡聚糖进行分离制备,β葡聚糖的提取率为2%。DNS法和考马斯亮蓝法检测提取品无还原糖和蛋白质。与标准的β葡聚糖进行比较,可作为底物对β葡聚糖酶活性测定。
The method of the preparation of β-glucan from barley was developed by enzyme treatment.The recovery of β-glucan separated reached 2%. Neither reducing sugar nor protein was determined by DNS and Bradford method respectively.There was no difference between β-glucan separated and Sigma's as substrate for determining the enzyme activity.
出处
《安徽农业科学》
CAS
北大核心
2005年第5期867-868,共2页
Journal of Anhui Agricultural Sciences
基金
安徽省教育厅自然科学基金(2003kj286)
合肥学院科研基金资助
关键词
大麦
Β-葡聚糖
分离纯化
β-葡聚糖酶活力
Barley
β-glucan, Separation and purifying
β-glucanase activity determination
