人类尾加压素Ⅱ在胎儿肺脏中分布的免疫组化研究

Immunohistochemical study on distribution of human urotensin Ⅱ in fetal lung tissue

目的为探索胎儿时期肺循环的调节机制,了解血管活性肽-人类尾加压素Ⅱ(hUⅡ)在胎儿肺脏中的表达情况。方法随机收集10例不同胎龄人工流产胎儿肺组织,常规福尔马林固定,石蜡包埋切片后用免疫组织化学技术,观察hUⅡ在肺组织中的表达。结果在胎龄12～15周的胎儿肺脏中,血管数目很少,免疫组化显示在血管组织中未见到免疫活性hUⅡ阳性表达,免疫活性hUⅡ在肺内支气管和细支气管的上皮中分布广泛;在胎龄18～25周的肺脏中,免疫活性hUⅡ表达显著减少,只在大型肺血管内皮和肺内支气管的上皮中表达。结论hUⅡ可能在人类肺脏的发生、分化过程中起一定的作用。

Objective Fetal pulmonary circulation was characterized by high resistance and low flow rate. But its mechanism was not fully understood. In order to explore the regulatory mechanism of pulmonary circulation, the distribution of immunoreactive human urotensin Ⅱ in the lung in different gestation periods was investigated. Methods The lung specimens from abortive fetuses(n= 10)in different gestational periods were randomly collected and fixed in formalin and embedded with paraffin. The expression level and distribution of human adrenomedullin urotensin Ⅱwere evaluated with immunohistochemistry technique. Results Urotensin Ⅱ was widely distributed in the epithelial cells of bronchi,with the peak level in the specimens from 12 to 15 week gestational period. While U Ⅱ immunostaining intensity was significantly decreased in the bronchial epithelial cells among those gestational period from 18 to 25 weeks. Only in relatively larger bronchi and blood vessels,could a little positive staining in the epithelial and endothelial cells could be observed. Conclusions Human urotensin Ⅱ existed in fetal lung and might play a role in ontogenesis.