灯盏花素对大鼠脑缺血再灌注后凋亡相关蛋白的干预

Breviscapine for the intervention of apoptosis-related protein in rats following cerebral ischemia-reperfusion

目的:探讨灯盏花素干预脑缺血再灌注损伤后对Bcl-2蛋白、c-Fos蛋白、半胱氨酸天冬氨酸蛋白酶3蛋白的表达影响及其对大脑损伤的保护作用。方法:实验于2004-06/09在华中科技大学同济医学院生理学实验室进行,取SD大鼠30只,随机分为假手术组、模型组和灯盏花组3组,每组10只。假手术组不造模,模型组和灯盏花组建立脑缺血再灌注模型后分别腹腔注射生理盐水1mL/kg和灯盏花素注射液1mL/kg。采用免疫组化法测定灯盏花素干预大鼠脑缺血24h后对Bcl-2蛋白、c-Fos蛋白、半胱氨酸天冬氨酸蛋白酶3蛋白表达的变化。结果:30只大鼠均进入结果分析。①Bcl-2蛋白:模型组大鼠脑组织中阳性细胞数与假手术组相比有显著差异犤(40.83±2.69),(2.02±0.18)个/视野,t=7.12,P<0.01犦,而灯盏花组又明显高于模型组犤(64.88±3.13)个/视野,t=9.34,P<0.01犦。②c-Fos蛋白:模型组大鼠脑组织中c-Fos蛋白表达水平显著高于假手术组犤(72.47±4.23),(2.30±0.34)个/视野,t=10.22,P<0.01犦;灯盏花组较模型组明显减少犤(48.23±5.12)个/视野,t=7.55,P<0.01犦。③半胱氨酸天冬氨酸蛋白酶3蛋白:模型组大鼠脑组织中阳性细胞数明显高于假手术组犤(126.31±7.12),(2.30±0.64)个/视野,t=15.74,P<0.01犦。

AIM:To study the effect of breviscapine on the expression of Bcl 2,c Fos and Caspase 3 proteins in rats with cerebral ischemia reperfusion injury and the protective role in brain. METHODS:The experiment was performed in the Department of Physiology,Tongji Medical College,Huazhong University of Science and Technology from June to September 2004.Thirty SD rats were divided randomly into three groups: sham operation group,model group and breviscapine group, with ten rats in each.After the establishment of cerebral ischemia reperfusion models,rats in the model group and breviscapine group were injected with 1 mL/kg saline and 1 mL/kg breviscapine into abdominal cavity, respectively.The expressions of Bcl 2, c Fos and Caspase 3 proteins were detected by using immunohistochemical method 24 hours after the treatment of cerebral ischemia reperfusion injury with breviscapine.RESULTS:All 30 rats were involved in the result analysis.① As compared with the model group (40.83± 2.69), the positive expression of Bcl 2 protein in brain tissue was significantly lower in the sham operation group (2.02± 0.18,t=7.12,P< 0.01),but was higher in the breviscapine group (64.88± 3.13,t=9.34,P< 0.01).② The positive expression of c Fos protein in brain tissue in model group(72.47± 4.23) increased obviously as compared with that in sham operation group(2.30± 0.34,t=10.22, P< 0.01) or in breviscapine group(48.23± 5.12,t=7.55,P< 0.01).③ The positive expression of Caspase 3 protein in brain tissue in model group(126.31± 7.12)increased obviously as compared with that in sham operation(2.30± 0.64,t=15.74, P< 0.01)or in breviscapine group (42.22± 4.24, t=7.36,PCONLUSION:Breviscapine may protect the brain through upregulating the levels of Bcl 2, c Fos and Caspase 3 proteins to inhibit neuron apoptosis following cerebral ischemia reperfusion injury.