韭菜花器官的组织培养

The tissue culture of flower organ in Chinese chive

田间韭菜植株上花苞苞膜未涨开时的花茎(韭薹)、花盘、花柄、花蕾等组织器官,在附加不同种类和浓度植物生长调节剂的MS培养基中,脱分化诱导生成愈伤组织、再分化成不定芽的能力不同。其中花盘切块在供试培养基中均高频率实现出愈和芽分化并长出新叶,花蕾和花柄受培养基中添加的BA、NAA影响较大,花茎始终无一分化。

There are different abilities of inducing to form calli and to differentiate buds by means of adding hormones into MS culture medium on flower organ, including floral axis, flower disc, anthocaulus and bud in Chinese Chive(Allium tuberosum Rottle.ex spr.). Among them, flower disc shows high frequency of callus formation and bud regeneration, but anthocaulus and bud are strongly effected by BA and NAA. However, floral axis never appears to have differentiation.