苯并[a]芘对肺腺癌细胞热休克蛋白70表达在细胞染色体损伤中作用的研究

Role study of benzo(a)pyrene on Heat shock proteins 70 expression of lung adenocarcinoma cell in cell chromosome damage

目的 探讨不同浓度苯并[a]芘(BaP)作用不同时间时,体外人类肺腺癌细胞热休克蛋白70 (HSP70 )表达在染色体损伤和细胞凋亡中的作用。方法 用Westernblot和AnnexinV -FITCapoptosis凋亡试剂盒以及松胞素B阻断核质分裂微核法分别研究了BaP不同浓度作用6h体外人类肺腺癌A5 4 9细胞HSP70的表达、细胞凋亡和染色体的损伤。结果 0～10μmol/L剂量范围内的BaP作用6h时,肺腺癌细胞HSP70的表达呈不断降低的趋势;细胞凋亡率升高,与对照组相比,染毒浓度为5～10 μmol/L时有显著性差异;细胞微核率升高,与对照组相比有显著性差异。结论 低浓度、短时间作用BaP的细胞毒性主要表现为对细胞遗传物质的损伤,细胞凋亡主要在高浓度的BaP诱导下发生,高表达的HSP70可以保护BaP致人类肺腺癌细胞染色体的损伤和凋亡。

Objective It is to discuss the role of different concentration and different times benzo(a)pyrene (BaP) on Heat shock proteins 70 (HSP70) expression of lung adenocarcinoma cell in vitro of mankind in chsomosome damage and apoptosis. Methods The roles of different concentration and 6 h BaP on HSP70 expression of lung adenocarcinoma cell A549 in vitro of mankind, apoptosis and chromosome damage were respectively studied through Western-blot, Annexin V-FITC apoptosis kit and cytokinesis-blocked micronucleus (CBMN) test. Results There was a decrease on HSP70 levels in lung adenocarcinoma cell A549 treated with 0～10?μmol/L BaP for 6?h. There was an advance on apoptosis rate and that in BaP group was higher at 5～10 μmol/L than that in control group. There was an advance on cell micronuclear rate and that in BaP group was higher than that in control group. Conclusion The cytotoxicity resulted by BaP at low concentration and short time mainly shows at inducing cytogenetic matter damage, and apoptosis is mainly induced by BaP at high concentration, high HSP70 expression can protect mankind lung adenocarcinoma cell chromosome damage and apoptosis induced by BaP.