胃癌癌变相关基因表达的cDNA微阵列研究

Gene expression profile of gastric tumorigenesis detected by cDNA microrray

目的 建立胃癌基因表达谱,筛选胃癌相关基因。方法 用含10 000个已知基因和7 000个ESTs(expressedsequencetags)的cDNA微阵列分析胃癌和癌旁正常胃黏膜基因表达谱的变化,半定量RT -PCR研究差异表达基因与胃癌的关系。结果 二倍以上的差异表达基因359个,其中在胃癌组织中表达上调271个,表达下调88个;二倍以上的差异表达ESTs28个,其中在胃癌组织中表达上调24个,下调4个。RT-PCR进一步证实碳酸酐酶Ⅱ在胃癌组织中存在表达下调,胰岛素样生长因子结合蛋白4在胃癌组织中存在表达上调。结论 发现碳酸酐酶Ⅱ、胰岛素样生长因子结合蛋白4可能与胃癌发生有关,为进一步寻找和克隆胃癌相关基因提供了重要的研究线索。

Purpose To establish the gene expression profiles of gastric carcinoma,and to identify gastric carcinoma-related genes.Methods The changes of gene expression profile between gastric carcinoma and adjacent normal tissue of gastric epithelia were analyzed by cDNA microarray which represents approximately 10 000 genes and 7 000 expressed sequence tags(ESTs) in this study. Reverse transcription polymerase chain reaction (RT-PCR) was employed to validate the relationship between gastric carcinoma-related genes and gastric carcinoma.Results 359 genes that were differentially expressed in cancer and non cancer tissues were identified; 271 genes were highly expressed>2.0-fold in cancer tissues,88 genes were poorly expressed >2.0-fold in cancer tissues,respectively; 28 ESTs that were differentially expressed in cancer and non cancer tissues were identified; 24 ESTs were highly expressed >2.0-fold in cancer tissues,4 ESTs were poorly expressed >2.0-fold in non cancer tissues. Furthermore,we found carbonic anhydrase Ⅱ was poorly expressed in gastric carcinoma,and insulin-like growth factor-binding protein 4 (IGFBP4) was highly expressed in gastric carcinoma using RT-PCR.Conclusion These results suggest that carbonic anhydrase Ⅱ and IGFBP4 may be associated with carcinogenesis of gastric carcinoma,and those also provide useful clues for future cloning gastric carcinoma related genes.