富硒乳酸菌对镉暴露机体自然杀伤细胞活性调节

Effect of se-enriched lactobacillus on NK cell activity in cadmium-poisoned rats

目的探讨镉暴露机体脾脏自然杀伤(NK)细胞活性变化及富硒乳酸菌对其的调节作用。方法72只健康成年雌性SD大鼠,随机分成4组,即对照组(C组)、低剂量镉染毒组(LCd组)、中剂量镉染毒组(MCd组)、高剂量镉染毒组(HCd组),C、LCd、MCd、HCd组分别饲喂含镉0,2,5,10mg/kg饲料的全价饲料进行镉暴露实验,连续9周。另选择48只健康成年雄性小鼠,随机分成C组、Cd组、镉染毒+中剂量富硒乳酸菌制剂保护组(CdMSe组)、镉染毒+高剂量富硒乳酸菌制剂保护组(CdHSe组),Cd、CdMSe、CdHSe组小鼠每天分别按含镉0.5mg/kg体重腹腔注射CdCl2溶液进行镉暴露,C组注射与Cd组同等剂量的生理盐水。连续12d。结果在整个实验期内,慢性镉暴露各组大鼠脾脏NK细胞活性均下降,且呈明显剂量时间反应关系;急性镉暴露小鼠NK细胞活性明显低于C组,而CdMSe组高于或显著高于Cd组,与C组接近,CdHSe组虽高于Cd组但低于C组和CdMSe组。结论急、慢性镉暴露均引起NK细胞活性下调,富硒乳酸菌能有效地对镉造成的机体损伤有保护作用,调节和改善NK细胞活性。

Objective To study the effect of Se-enriched lactobacillus on spleen NK cell activity in cadmium-poisoned rats.Methods Seventy-two healthy Sprague-Dawley female rats were chosen and divided into a control group(group C),a cadmium exposure group of low dose (group L-Cd),a cadmium exposure group of medium dose(group M-Cd)and a cadmium exposure group of high dose(group H-Cd).group C,L-Cd,M-Cd,H-Cd were orally dosed daily with 0,2,5,10mg/(kg·bw) cadmium for 9 weeks,respectively.Forty-eight healthy ICR male mice were chosen and divided randomly into a control group(group C),a cadmium exposure group (group Cd),a cadmium plus medium dose Se-enriched lactobacillus group(group Cd-MSe)and a cadmium plus high dose Se-enriched lactobacills group(group Cd-HSe).Groups Cd,Cd-MSe and Cd-HSe were injected in the abdominal cavity with 0.5mg/(kg·bw) cadmium and group C with saline of the same dosage in group Cd for 12 days.Results During the entire experimental period,spleen NK cell activity of all groups with chronic cadmium exposure decreased,and displayed a relationship which intensified obviously with dosage increases and time elongation of cadmium exposure.The spleen NK cell activity of group Cd with acute cadmium exposure decreased significantly as compared with group C.The activity of group Cd-MSe was close to group C and superior of significantly higher than that of group Cd.The activity of group Cd-HSe was higher than that of group Cd,but lower than that of group C and Cd-MSe.Conclusion Acute and chronic cadmium exposure induced downregulation of NK cell activity.Se-enriched lactobacillus could protect effectively the injury induced by cadmium,and adjusted and ameliorated NK cell activity.