电磁辐射对大鼠海马MAPK信号通路的影响

Effects of electromagnetic irradiation on MAPK singal transduction pathway of hippocampus neuron in rats

目的观察电磁辐射对大鼠海马神经细胞丝裂原活化蛋白激酶(MAPK)家族基因及蛋白质表达水平的影响。方法以65mW/cm2电磁波急性辐照大鼠20min,采用RTPCR检测细胞外的调节蛋白激酶1(ERK1)、CJun氨基未端激酶1(JNK1)、P38MAPKmRNA表达,采用蛋白质免疫印迹方法检测ERK1、JNK1、P38MAPK蛋白质表达。结果电磁波辐照后大鼠海马ERK1与JNK1的mRNA表达及蛋白质表达均明显增加,ERK1的高表达持续至辐照后12h,JNK1持续至辐照后3,24h后两者均不同程度低于对照水平,辐照后P38MAPK的mRNA及蛋白质表达变化不明显。结论电磁辐射可在基因转录和蛋白质表达水平对ERK和JNK2条信号转导通路产生影响,对P38MAPK的影响不明显。ERK和JNK两条信号转导通咱可能参与了电磁辐射导致的中枢神经损伤。

Objective To observe the influence of electromagnetic irradiation on mRNA and protein expression of MAPKs families in the hippocampus neuron of rats.Methods The rats were acute exposed to 65mW/cm2 electromagnetic wave 20min.The mRNA expressions of extracellular signal-regulated 1(ERK1),c-Jun N-terminal kinase1(JNK1),P38 MAPK were tested by RT-PCR.The protein expressions of ERK1,JNK1,P38 MAPK were tested by Western-blot.Results Ater electromagnetic irradiation,the mRNA and protein expressions of ERK1 and JNK1 increased significantly in the rat hippocampus.The high expression of ERK1 lasted 12 hours.But JNK1 expressed highly only for 3 hours.AT 24 hours after irradiation,the expression of ERK1 and JNK1 decreased below to the level of control.The mRNA and protein expressions of P38 MAPK didn't change obviously.Conclusion The electromagnetic irradiation can influence ERK and JNK signal transduction in gene transcription and protein expression.But it has no influences on P38 MAPK signal transduction.The ERK and JNK signal transduction may play an important role in the center nerve system induced by electromagnetic irradiation.